7E4N
Crystal structure of Sat1646
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-15 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 89.017, 89.017, 68.674 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.610 - 1.940 |
| R-factor | 0.1979 |
| Rwork | 0.195 |
| R-free | 0.24730 |
| Structure solution method | SAD |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.950 | 1.990 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.111 | 1.625 |
| Number of reflections | 18106 | 905 |
| <I/σ(I)> | 18 | |
| Completeness [%] | 87.0 | |
| Redundancy | 21.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 15% v/v Tacsimate TM pH 7.0, 0.1M HEPES pH 7.0, 2% w/v Polyethylene glycol 3350 |
