7E4M
Class I Pimarane-Type Diterpene Synthases Stt4548
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-15 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 66.870, 64.908, 70.583 |
| Unit cell angles | 90.00, 108.75, 90.00 |
Refinement procedure
| Resolution | 66.840 - 1.570 |
| R-factor | 0.1804 |
| Rwork | 0.179 |
| R-free | 0.20890 |
| Structure solution method | SAD |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.840 | 1.680 |
| High resolution limit [Å] | 1.570 | 1.580 |
| Rmerge | 0.067 | |
| Rpim | 0.468 | |
| Number of reflections | 31104 | 1555 |
| <I/σ(I)> | 13.4 | |
| Completeness [%] | 90.0 | |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.1M Sodium chloride, 0.1M BIS-TRIS pH 6.5, 1.5M Ammonium sulfate |
