7E3J

Crystal structure of SARS-CoV-2 RBD binding to dog ACE2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-08
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979
Spacegroup name	I 4 2 2
Unit cell lengths	168.256, 168.256, 211.196
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.260 - 2.990
R-factor	0.2282
Rwork	0.227
R-free	0.24790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6lzg
RMSD bond length	0.002
RMSD bond angle	0.531
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.110
High resolution limit [Å]	2.990	3.000
Number of reflections	30355	2989
<I/σ(I)>	8.8	2.6
Completeness [%]	98.5
Redundancy	6.3
CC(1/2)	0.980	0.819

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291	2% 1,4-dioxane, 0.1 M tris pH 8.0,15% polyethylene glycol 3,350.