7E2U
Synechocystis GUN4 in complex with phytochrome
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-12-20 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 32.522, 64.601, 106.892 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.180 - 1.700 |
R-factor | 0.1593 |
Rwork | 0.158 |
R-free | 0.19400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4xkc |
Data reduction software | HKL-3000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.111 | 0.712 |
Rpim | 0.046 | 0.340 |
Number of reflections | 25494 | 2325 |
<I/σ(I)> | 16.8 | 2.1 |
Completeness [%] | 99.1 | |
Redundancy | 6.4 | |
CC(1/2) | 0.989 | 0.688 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | Sodium fluoride, PEG 3350 |