7E2S

Synechocystis GUN4 in complex with biliverdin IXa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	32.497, 64.449, 106.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.130 - 1.050
R-factor	0.1185
Rwork	0.118
R-free	0.13430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xkb
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.090
High resolution limit [Å]	1.050	1.050
Rmerge	0.089	0.477
Rpim	0.027	0.158
Number of reflections	105355	10208
<I/σ(I)>	23.2	3.9
Completeness [%]	99.8
Redundancy	12
CC(1/2)	0.997	0.916

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	HEPES pH 7.0, Jeffamine M-600