7E2R
The ligand-free structure of Arabidopsis thaliana GUN4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-04-18 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.758, 64.118, 55.705 |
Unit cell angles | 90.00, 91.59, 90.00 |
Refinement procedure
Resolution | 42.040 - 2.300 |
R-factor | 0.1961 |
Rwork | 0.195 |
R-free | 0.22480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4xkb |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.140 | 0.813 |
Rpim | 0.084 | 0.486 |
Number of reflections | 15049 | 1495 |
<I/σ(I)> | 9 | |
Completeness [%] | 99.8 | 100 |
Redundancy | 3.7 | |
CC(1/2) | 0.981 | 0.651 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | Bis-Tris, Polythylene glycol monomethyl ether |