7E2Q
Crystal structure of Mycoplasma pneumoniae Enolase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-12-19 |
Detector | AGILENT ATLAS CCD |
Wavelength(s) | 0.97853 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 76.074, 106.548, 128.589 |
Unit cell angles | 90.00, 103.15, 90.00 |
Refinement procedure
Resolution | 19.840 - 1.800 |
R-factor | 0.1909 |
Rwork | 0.189 |
R-free | 0.22260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6j36 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.787 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.840 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 183711 | 26642 |
<I/σ(I)> | 6.4 | |
Completeness [%] | 99.6 | |
Redundancy | 6.4 | |
CC(1/2) | 0.997 | 0.760 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2M Sodium citrate tribasic dihydrate, 0.1M TRIS hydrochloride pH 8.5, 30% v/v Polyethylene glycol 400 |