7E2M

Crystal structure of the RWD domain of human GCN2 - 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97895
Spacegroup name	P 63
Unit cell lengths	158.440, 158.440, 54.500
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.901 - 2.350
R-factor	0.2358
Rwork	0.234
R-free	0.26110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7e2k
Data scaling software	Aimless (0.7.2)
Refinement software	PHENIX (1.13)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.901	44.900	2.410
High resolution limit [Å]	2.350	10.510	2.350
Rmerge	0.193	0.082	1.301
Rmeas	0.202	0.086	1.368
Rpim	0.060	0.027	0.419
Total number of observations		4034	25379
Number of reflections	63134	391	2376
<I/σ(I)>	10.9	18	2.5
Completeness [%]	99.3	96	99.4
Redundancy	11.2	10.3	10.7
CC(1/2)	0.994	0.998	0.399

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M sodium citrate pH 7.0, 20 % PEG4000, and 20 % 2-propanol