7E2K

Crystal structure of the RWD domain of human GCN2 - 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97895
Spacegroup name	I 2 3
Unit cell lengths	110.370, 110.370, 110.370
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.058 - 2.041
R-factor	0.2164
Rwork	0.215
R-free	0.23290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ukx
Data scaling software	Aimless (0.7.2)
Refinement software	PHENIX (1.13)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.060	45.060	2.090
High resolution limit [Å]	2.040	9.130	2.040
Rmerge	0.136	0.057	1.595
Rmeas	0.140	0.059	1.631
Rpim	0.031	0.014	0.340
Total number of observations		3292	24214
Number of reflections	27621	188	1056
<I/σ(I)>	15.7	40.8	2.3
Completeness [%]	100.0	99.5	100
Redundancy	21.4	17.5	22.9
CC(1/2)	0.999	0.999	0.663

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	1.6 M sodium citrate tribasic dihydrate pH 6.5