7E2B
GDPD mutant complex from Pyrococcus furiosus DSM 3638
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-11-29 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97921 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.920, 70.120, 90.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.830 - 1.850 |
R-factor | 0.1843 |
Rwork | 0.181 |
R-free | 0.22010 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4oec |
RMSD bond length | 0.014 |
RMSD bond angle | 1.369 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.120 | 1.900 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.124 | |
Rpim | 0.053 | |
Number of reflections | 26190 | 1875 |
<I/σ(I)> | 13.4 | |
Completeness [%] | 99.9 | |
Redundancy | 11.6 | |
CC(1/2) | 0.997 | 0.534 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.1M Hepes (pH 7.5), 25% w/v PEG3350, 0.2M Ammonium sulfate |