7E29
Crystal Structure of Saccharomyces cerevisiae Ioc4 PWWP domain fused with MBP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-09-21 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | P 63 |
Unit cell lengths | 153.303, 153.303, 42.004 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 32.209 - 2.303 |
R-factor | 0.1771 |
Rwork | 0.173 |
R-free | 0.22880 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hsj |
RMSD bond length | 0.008 |
RMSD bond angle | 1.117 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.109 | 0.706 |
Number of reflections | 25377 | 1261 |
<I/σ(I)> | 35.5 | |
Completeness [%] | 99.9 | |
Redundancy | 7.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | Potassium thiocyanate, Polyethylene glycol monomethyl ether 2000 |