7E1T

Crystal structure of Rab9A-GTP-Nde1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-07
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9785
Spacegroup name	P 21 21 21
Unit cell lengths	67.421, 67.646, 118.516
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.750 - 2.450
R-factor	0.1962
Rwork	0.194
R-free	0.24380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4qxa
RMSD bond length	0.008
RMSD bond angle	1.202
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0123)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.750	2.510
High resolution limit [Å]	2.450	2.450
Rmerge	0.170
Number of reflections	18238	912
<I/σ(I)>	8.5	2
Completeness [%]	88.5	65.2
Redundancy	5.4
CC(1/2)	0.991	0.715

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	5.5	290	0.1 M Bis-Tris, pH 5.5 and 25% PEG 3350.