7E1K

Crystal structure of Pr55Gag-matrix domain in complex with IP6 in space group R32

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-02
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.10
Spacegroup name	H 3 2
Unit cell lengths	97.828, 97.828, 176.297
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.100 - 2.400
R-factor	0.2233
Rwork	0.220
R-free	0.27590
Structure solution method	FOURIER SYNTHESIS
Data reduction software	XDS
Data scaling software	Aimless (0.5.31)
Phasing software	PHENIX
Refinement software	PHENIX (DEV-3318)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.910	48.910	2.490
High resolution limit [Å]	2.400	8.980	2.400
Rmerge	0.217	0.042	1.707
Rmeas	0.222	0.044	1.749
Rpim	0.049	0.010	0.379
Total number of observations	263912	4963	28133
Number of reflections	12979	280	1329
<I/σ(I)>	12	40.9	2.2
Completeness [%]	99.9	98.9	100
Redundancy	20.3	17.7	21.2
CC(1/2)	0.998	1.000	0.860

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	PEG 3350 and 100 mM MES