7E1I
Crystal structure of Pr55Gag-matrix domain in complex with IP6 at ambient temperature in space group P1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SLAC LCLS BEAMLINE CXI |
| Synchrotron site | SLAC LCLS |
| Beamline | CXI |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2018-12-01 |
| Detector | CS-PAD CXI-1 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 96.600, 96.600, 91.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.574 - 3.301 |
| R-factor | 0.3534 |
| Rwork | 0.351 |
| R-free | 0.40840 |
| Structure solution method | FOURIER SYNTHESIS |
| Data reduction software | CrystFEL |
| Data scaling software | CrystFEL |
| Phasing software | PHENIX |
| Refinement software | PHENIX (DEV-3318) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.000 |
| High resolution limit [Å] | 3.300 |
| Number of reflections | 35663 |
| <I/σ(I)> | 2.67 |
| Completeness [%] | 100.0 |
| Redundancy | 62.3 |
| CC(1/2) | 0.958 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 20% (w/v) polyethylene glycol 3350 (PEG 3350) as a precipitant and 100 mM MES-NaOH |
