7E1G
Structure of MreB3 from Spiroplasma eriocheiris
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-29 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.290, 56.270, 120.531 |
Unit cell angles | 90.00, 90.56, 90.00 |
Refinement procedure
Resolution | 50.987 - 1.750 |
R-factor | 0.1756 |
Rwork | 0.174 |
R-free | 0.21350 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7e1c |
RMSD bond length | 0.006 |
RMSD bond angle | 0.891 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.15.2_3472: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 56.300 | 1.780 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.101 | 0.570 |
Rpim | 0.065 | 0.372 |
Number of reflections | 66116 | 3545 |
<I/σ(I)> | 6.8 | 1.8 |
Completeness [%] | 97.2 | |
Redundancy | 3.3 | |
CC(1/2) | 0.993 | 0.658 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.8 | 293 | 30% (w/v) PEG 4000, 0.1 M Acetate-NaOH pH 4.6, 200 mM NH4Ac, 5 mM AMPPNP, 5mM MgCl2 |