7E1F
Native-DBD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97890 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.455, 52.135, 96.961 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.318 - 1.447 |
| R-factor | 0.1917 |
| Rwork | 0.190 |
| R-free | 0.21630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Se-DBD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.809 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.500 |
| High resolution limit [Å] | 1.447 | 1.450 |
| Number of reflections | 29399 | 29399 |
| <I/σ(I)> | 37.34 | |
| Completeness [%] | 97.9 | |
| Redundancy | 12.2 | |
| CC(1/2) | 0.994 | 0.979 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M NaCl, 0.1 M HEPES pH 7.0, and 20% (W/V) PEG6000 |
