7E1C
Structure of MreB3 from Spiroplasma eriocheiris
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-26 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.420, 68.090, 54.570 |
| Unit cell angles | 90.00, 91.73, 90.00 |
Refinement procedure
| Resolution | 54.540 - 1.900 |
| R-factor | 0.1878 |
| Rwork | 0.185 |
| R-free | 0.22130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4czl |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.614 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.540 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.094 | 0.542 |
| Rpim | 0.045 | 0.263 |
| Number of reflections | 30343 | 1962 |
| <I/σ(I)> | 9.8 | 2.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.3 | 5.2 |
| CC(1/2) | 0.996 | 0.866 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | 20% (w/v) PEG 8000, 0.1 M MES-NaOH pH 6.0, 200 mM Ca(OAc)2 |
