7E1C

Structure of MreB3 from Spiroplasma eriocheiris

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000
Spacegroup name	P 1 21 1
Unit cell lengths	52.420, 68.090, 54.570
Unit cell angles	90.00, 91.73, 90.00

Refinement procedure

Resolution	54.540 - 1.900
R-factor	0.1878
Rwork	0.185
R-free	0.22130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4czl
RMSD bond length	0.004
RMSD bond angle	0.614
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.540	1.940
High resolution limit [Å]	1.900	1.900
Rmerge	0.094	0.542
Rpim	0.045	0.263
Number of reflections	30343	1962
<I/σ(I)>	9.8	2.7
Completeness [%]	100.0	100
Redundancy	5.3	5.2
CC(1/2)	0.996	0.866

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	277	20% (w/v) PEG 8000, 0.1 M MES-NaOH pH 6.0, 200 mM Ca(OAc)2