7E15
Protein ternary complex working for DNA replication initiation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-29 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 69.302, 48.308, 205.759 |
Unit cell angles | 90.00, 96.19, 90.00 |
Refinement procedure
Resolution | 47.010 - 2.450 |
R-factor | 0.2174 |
Rwork | 0.216 |
R-free | 0.24190 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ghr |
RMSD bond length | 0.002 |
RMSD bond angle | 0.477 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.490 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.078 | 0.398 |
Rmeas | 0.110 | 0.560 |
Rpim | 0.077 | 0.395 |
Number of reflections | 93014 | 16322 |
<I/σ(I)> | 6.9 | 2 |
Completeness [%] | 99.5 | 99.7 |
Redundancy | 3.5 | 3.5 |
CC(1/2) | 0.997 | 0.927 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.15 M DL-malic acid (pH 7.0), 20 % (w/v) PEG 3350 |