7E15
Protein ternary complex working for DNA replication initiation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-29 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.302, 48.308, 205.759 |
| Unit cell angles | 90.00, 96.19, 90.00 |
Refinement procedure
| Resolution | 47.010 - 2.450 |
| R-factor | 0.2174 |
| Rwork | 0.216 |
| R-free | 0.24190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ghr |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.477 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.078 | 0.398 |
| Rmeas | 0.110 | 0.560 |
| Rpim | 0.077 | 0.395 |
| Number of reflections | 93014 | 16322 |
| <I/σ(I)> | 6.9 | 2 |
| Completeness [%] | 99.5 | 99.7 |
| Redundancy | 3.5 | 3.5 |
| CC(1/2) | 0.997 | 0.927 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.15 M DL-malic acid (pH 7.0), 20 % (w/v) PEG 3350 |
