7E0W

Crystal Structure of BCH domain from S. pombe

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-15
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9789
Spacegroup name	P 61
Unit cell lengths	108.390, 108.390, 250.410
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.736 - 2.800
R-factor	0.2317
Rwork	0.228
R-free	0.26030
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.838
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.930
High resolution limit [Å]	2.800	2.800
Number of reflections	36910	2453
<I/σ(I)>	12.6
Completeness [%]	87.7
Redundancy	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.1M Bis-Tris propane pH 7.0 and 2.1M NaCl.