7E0N
Crystal structure of Monoacylglycerol Lipase chimera
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-17 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 64.798, 99.854, 207.236 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.630 - 1.850 |
| R-factor | 0.1552 |
| Rwork | 0.155 |
| R-free | 0.17790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5xks |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.149 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.18.1_3865) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.124 | 0.802 |
| Rpim | 0.054 | 0.234 |
| Number of reflections | 115284 | 5671 |
| <I/σ(I)> | 19.5 | 4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.9 | 11.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | potassium phosphate dibasic, sodium phosphate monobasic, imidazole/hydrochloric acid |
