7E0D

Structure of L-glutamate oxidase R305E mutant in complex with L-arginine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-11-09
Detector	RAYONIX MX225HE
Wavelength(s)	1.000
Spacegroup name	P 61 2 2
Unit cell lengths	124.760, 124.760, 170.201
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	54.000 - 2.700
R-factor	0.2009
Rwork	0.198
R-free	0.25510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2e1m
RMSD bond length	0.002
RMSD bond angle	0.549
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.15.2_3472: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.000	2.830
High resolution limit [Å]	2.700	2.700
Rmerge	0.178	0.699
Rpim	0.089	0.317
Number of reflections	21123	2842
<I/σ(I)>	7.7	2.5
Completeness [%]	96.4	99.1
Redundancy	4.8	4.8
CC(1/2)	0.981	0.744

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	12% (w/v) PEG 8000, 0.1M Tris-HCl pH7.0, 75mM MgCl2