7E0C

Structure of L-glutamate oxidase R305E mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.000
Spacegroup name	P 61 2 2
Unit cell lengths	123.970, 123.970, 168.631
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	66.311 - 2.650
R-factor	0.1934
Rwork	0.191
R-free	0.24090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2e1m
RMSD bond length	0.006
RMSD bond angle	0.860
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.15.2_3472: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	84.300	2.780
High resolution limit [Å]	2.650	2.650
Rmerge	0.115	0.670
Rpim	0.034	0.191
Number of reflections	22897	2970
<I/σ(I)>	18.5	4.6
Completeness [%]	99.9	100
Redundancy	12.3	13
CC(1/2)	0.999	0.930

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	12% (w/v) PEG 8000, 0.1M Tris-HCl pH7.0, 75mM MgCl2