7E0B

The crystal structure of sorting nexin 27 and PBM complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-04-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9791
Spacegroup name	H 3
Unit cell lengths	88.550, 88.550, 43.270
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.820 - 1.290
R-factor	0.1845
Rwork	0.184
R-free	0.19060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4z8j
RMSD bond length	0.003
RMSD bond angle	0.543
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.140	1.320
High resolution limit [Å]	1.290	1.290
Rmerge	0.055
Number of reflections	30746	6895
<I/σ(I)>	17.3
Completeness [%]	97.8
Redundancy	8.6
CC(1/2)	0.998	0.519

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291	0.2 M sodium sulfate, 20 % w/v PEG 3350.