7E09
Trans-3/4-proline-hydroxylase H11 with 3-hydroxyl-proline
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-12-10 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979 |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 75.390, 75.390, 143.650 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 75.390 - 2.380 |
R-factor | 0.1933 |
Rwork | 0.192 |
R-free | 0.22550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6LNS |
RMSD bond length | 0.018 |
RMSD bond angle | 1.898 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 75.390 | 2.420 |
High resolution limit [Å] | 2.380 | 2.380 |
Rmerge | 0.067 | 0.502 |
Rmeas | 0.070 | 0.521 |
Rpim | 0.020 | 0.141 |
Number of reflections | 17231 | 856 |
<I/σ(I)> | 86.4 | 10.3 |
Completeness [%] | 99.9 | 100 |
Redundancy | 12.6 | 13.2 |
CC(1/2) | 0.969 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 0.7 | 289.15 | 0.7M Sodium citrate tribasic dihydrate, 0.1M Bis-Tris propane (pH 7.0) |