7E07

Trans-3/4-proline-hydroxylase H11 with AKG and L-proline

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2017-06-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 41 21 2
Unit cell lengths	106.437, 106.437, 145.016
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	85.810 - 2.140
R-factor	0.1907
Rwork	0.189
R-free	0.23320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6LNS
RMSD bond length	0.019
RMSD bond angle	1.963
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	85.810	2.180
High resolution limit [Å]	2.140	2.140
Rmerge	0.132	0.529
Rmeas	0.135	0.538
Rpim	0.025	0.100
Number of reflections	44086	2262
<I/σ(I)>	8.3	1.3
Completeness [%]	94.5	99
Redundancy	28.6	28.9
CC(1/2)		0.981

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	289.15	0.7M Sodium citrate tribasic dihydrate, 0.1M Bis-Tris propane (pH 7.0)