7E03

Room temperature structure of glucose isomerase delivered in beef tallow by serial millisecond crystallography

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	293.15
Detector technology	PIXEL
Collection date	2019-05-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9796
Spacegroup name	I 2 2 2
Unit cell lengths	94.330, 99.910, 103.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	71.820 - 1.600
R-factor	0.1686
Rwork	0.168
R-free	0.18620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5y4i
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	72.460	1.660
High resolution limit [Å]	1.600	1.600
Number of reflections	64497	6368
<I/σ(I)>	4.79	1.52
Completeness [%]	100.0
Redundancy	250.9
CC(1/2)	0.966	0.535

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	7	293.5	Tris-HCl, Ammonium sulfate, Magnesium sulfate