7E02

Room temperature structure of lysozyme delivered in beef tallow by serial millisecond crystallography

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	293.15
Detector technology	PIXEL
Collection date	2019-05-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9796
Spacegroup name	P 43 21 2
Unit cell lengths	79.400, 79.400, 38.420
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.140 - 1.550
R-factor	0.1699
Rwork	0.168
R-free	0.18790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7cvj
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.000	1.600
High resolution limit [Å]	1.550	1.550
Number of reflections	18428	1809
<I/σ(I)>	10.63	1.87
Completeness [%]	100.0	100
Redundancy	1257.8	667.4
CC(1/2)	0.994	0.624

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES	4.5	293.5	sodium acetate, PEG 8000, NaCl