7E00
Trans-proline-hydroxylase H11 with Succinic and L-proline in the fourth reaction state.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-10-26 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 106.367, 106.367, 143.924 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 85.540 - 1.920 |
Rwork | 0.195 |
R-free | 0.23040 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7dt0 |
Data reduction software | DIALS |
Data scaling software | DIALS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 85.540 | 2.020 |
High resolution limit [Å] | 1.920 | 1.920 |
Rmerge | 0.136 | 1.218 |
Rpim | 0.351 | |
Number of reflections | 63525 | 9204 |
<I/σ(I)> | 11.2 | |
Completeness [%] | 99.2 | 100 |
Redundancy | 13 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289.15 | 0.7 M Sodium citrate tribasic dihydrate, 0.1 M Bis-Tris propane (pH 7.0) |