7DZL
A69C-M71L mutant of Fabp protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-30 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.987, 66.830, 68.406 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.850 - 1.640 |
R-factor | 0.1884 |
Rwork | 0.187 |
R-free | 0.21500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1lkk |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.450 | 1.720 |
High resolution limit [Å] | 1.630 | 1.630 |
Rmerge | 0.031 | 0.123 |
Number of reflections | 32859 | 3185 |
<I/σ(I)> | 38.98 | 10.8 |
Completeness [%] | 99.7 | 98.8 |
Redundancy | 7.5 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 0.1 M potassium thiocyanate, pH 7.0, 30% PEG 2000 monomethyl ether |