7DZ2
Crystal structures of D-allulose 3-epimerase from Sinorhizobium fredii
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
Synchrotron site | Photon Factory |
Beamline | AR-NE3A |
Temperature [K] | 95 |
Detector technology | PIXEL |
Collection date | 2019-12-07 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 62.973, 88.297, 128.695 |
Unit cell angles | 90.00, 99.29, 90.00 |
Refinement procedure
Resolution | 32.650 - 1.550 |
R-factor | 0.1702 |
Rwork | 0.169 |
R-free | 0.18770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5zfs |
RMSD bond length | 0.006 |
RMSD bond angle | 0.857 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.170 | 1.580 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmeas | 0.062 | 0.062 |
Rpim | 0.023 | 0.428 |
Number of reflections | 195342 | 9475 |
<I/σ(I)> | 17.6 | 2 |
Completeness [%] | 97.2 | |
Redundancy | 6.9 | |
CC(1/2) | 0.999 | 0.743 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M Lithium sulfate monohydrate, 0.1 M BIS-TRIS pH 6.5, 25% w/v PEG 3,350 |