7DYK
Crystal Structure of Cyanobacterial Circadian Clock Protein KaiC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-07 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 63 |
| Unit cell lengths | 94.847, 94.847, 180.560 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.550 - 2.990 |
| R-factor | 0.2764 |
| Rwork | 0.274 |
| R-free | 0.32790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7dyj |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.223 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.550 | 3.110 |
| High resolution limit [Å] | 2.990 | 3.000 |
| Rmerge | 0.138 | 0.962 |
| Number of reflections | 19313 | 1788 |
| <I/σ(I)> | 12.9 | |
| Completeness [%] | 99.7 | |
| Redundancy | 10.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7 | 313 | sodium/pottasium tartrae, sodium acetate, KCl |
