7DYJ

Crystal Structure of Cyanobacterial Circadian Clock Protein KaiC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2015-09-30
Detector	RAYONIX MX-225
Wavelength(s)	0.9
Spacegroup name	P 63
Unit cell lengths	94.582, 94.582, 179.858
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.290 - 2.400
R-factor	0.2687
Rwork	0.267
R-free	0.31020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gbl
RMSD bond length	0.002
RMSD bond angle	1.248
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0253)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.440
High resolution limit [Å]	2.400	2.400
Number of reflections	35578	1775
<I/σ(I)>	36.4
Completeness [%]	100.0
Redundancy	14.3
CC(1/2)	0.997	0.793

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7	313	sodium/pottasium tartrae, sodium acetate, KCl