7DYE
Crystal Structure of Cyanobacterial Circadian Clock Protein KaiC
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-04-09 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9 |
Spacegroup name | H 3 |
Unit cell lengths | 94.906, 94.906, 276.523 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.500 - 2.600 |
R-factor | 0.2819 |
Rwork | 0.279 |
R-free | 0.33660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2gbl |
RMSD bond length | 0.003 |
RMSD bond angle | 1.249 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.500 | 2.720 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.069 | 0.612 |
Number of reflections | 28555 | 3485 |
<I/σ(I)> | 12.5 | |
Completeness [%] | 99.9 | |
Redundancy | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 7 | 313 | sodium/potassium tartrate, sodium acetate, KCl |