7DY9

Thermotoga maritima ferritin mutant-FLAL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL18U
Synchrotron site	NFPSS
Beamline	BL18U
Temperature [K]	100
Detector technology	CCD
Collection date	2019-11-04
Detector	RAYONIX MX-225
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	38.305, 83.399, 103.802
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.065 - 2.303
R-factor	0.2959
Rwork	0.291
R-free	0.38580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vlg
RMSD bond length	0.011
RMSD bond angle	1.205
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	2.640
High resolution limit [Å]	2.303	2.303
Number of reflections	14942	9361
<I/σ(I)>	6.5
Completeness [%]	95.2
Redundancy	7.6
CC(1/2)	0.997	0.935

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	30% PEG 1500