7DY3
High resolution crystal structure of hemoglobin M Iwate.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-10 |
| Detector | DECTRIS PILATUS3 X 200K-A |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.494, 86.275, 121.005 |
| Unit cell angles | 90.00, 99.25, 90.00 |
Refinement procedure
| Resolution | 13.080 - 1.400 |
| R-factor | 0.1695 |
| Rwork | 0.168 |
| R-free | 0.19290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dn2 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.108 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 13.080 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 228772 | 862680 |
| <I/σ(I)> | 17.9 | |
| Completeness [%] | 98.4 | |
| Redundancy | 3.8 | |
| CC(1/2) | 0.998 | 0.780 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 25%w/v PEG 3350, 0.1M HEPES-NaOH buffer, 0.2M lithium sulfate, pH 7.5 |
