7DXY

Crystal structure of the chemically synthesized mk2h_deltaMILPS peptide homodimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	30.660, 39.688, 64.283
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.263 - 1.400
R-factor	0.2129
Rwork	0.211
R-free	0.22960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7dxx
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.440
High resolution limit [Å]	1.350	1.350
Number of reflections	17566	2759
<I/σ(I)>	18.85
Completeness [%]	99.2
Redundancy	6.26
CC(1/2)	1.000	0.832

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	400mM Sodium phosphate monobasic/1600mM Pottasium phosphate dibasic, 100 mM imidazole_HCl, pH 8.0, 200 mM NaCl