7DXV
Crystal structure of the mk2h_deltaY peptide homodimer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2020-07-22 |
Detector | RAYONIX MX225-HS |
Wavelength(s) | 1 |
Spacegroup name | H 3 |
Unit cell lengths | 73.101, 73.101, 49.177 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 36.551 - 2.301 |
R-factor | 0.2061 |
Rwork | 0.202 |
R-free | 0.24830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7dxr |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.340 |
High resolution limit [Å] | 2.210 | 2.210 |
Number of reflections | 4833 | 705 |
<I/σ(I)> | 29.72 | |
Completeness [%] | 98.0 | |
Redundancy | 10.87 | |
CC(1/2) | 0.999 | 0.993 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM SPG buffer, 25 % PEG 1500 |