7DXQ
Crystal Structure of Cyanobacterial Circadian Clock Protein KaiC
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-05-30 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.9 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 92.411, 159.874, 207.243 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.960 - 2.800 |
R-factor | 0.2532 |
Rwork | 0.250 |
R-free | 0.31760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2gbl |
RMSD bond length | 0.002 |
RMSD bond angle | 1.193 |
Data reduction software | HKL-3000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.092 | |
Number of reflections | 75955 | 7451 |
<I/σ(I)> | 21.4 | |
Completeness [%] | 99.9 | |
Redundancy | 7.4 | |
CC(1/2) | 0.751 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 5 | 313 | Acetatic acid, Sodium formate |