7DXN

Plant growth-promoting factor YxaL from Bacillus velezensis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100.15
Detector technology	CCD
Collection date	2019-04-23
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97934
Spacegroup name	P 32 2 1
Unit cell lengths	81.524, 81.524, 195.905
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.760 - 1.800
R-factor	0.154
Rwork	0.153
R-free	0.18580
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.856
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.830
High resolution limit [Å]	1.800	1.800
Rmerge	0.070	0.336
Rpim	0.018	0.098
Number of reflections	70991	3512
<I/σ(I)>	39	6
Completeness [%]	99.9	100
Redundancy	14.3
CC(1/2)	0.998	0.907

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	289.15	0.1 M CHES-NaOH, 1 M sodium citrate