7DWR
Structure of Sulfolobus solfataricus SegA-ADP complex bound to DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE TPS 05A |
Synchrotron site | NSRRC |
Beamline | TPS 05A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-03-07 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 220.000, 60.541, 132.143 |
Unit cell angles | 90.00, 127.01, 90.00 |
Refinement procedure
Resolution | 27.680 - 2.800 |
R-factor | 0.2428 |
Rwork | 0.239 |
R-free | 0.29800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7dut |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 2.850 |
High resolution limit [Å] | 2.800 | 7.560 | 2.800 |
Rmerge | 0.107 | 0.043 | 0.450 |
Rmeas | 0.127 | 0.053 | 0.532 |
Rpim | 0.066 | 0.031 | 0.281 |
Total number of observations | 117429 | ||
Number of reflections | 32772 | 1667 | 1595 |
<I/σ(I)> | 6.9 | ||
Completeness [%] | 94.4 | 92 | 92.4 |
Redundancy | 3.6 | 3 | 3.4 |
CC(1/2) | 0.995 | 0.856 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | MES sodium salt pH 6.5, PEG8000, calcium acetate. |