7DWO

Crystal structure of Vibrio fischeri DarR in complex with DNA reveals the transcriptional activation mechanism of LTTR family members

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 2
Unit cell lengths	74.863, 180.223, 51.657
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.560 - 2.611
R-factor	0.2342
Rwork	0.233
R-free	0.26450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ydw
Data reduction software	DIALS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.890	29.890	2.680
High resolution limit [Å]	2.610	11.670	2.610
Rmerge	0.100	0.036	0.435
Rmeas	0.105	0.039	0.454
Rpim	0.029	0.013	0.129
Total number of observations	276628	2485	19476
Number of reflections	22031	289	1580
<I/σ(I)>	18.5	52.3	1.7
Completeness [%]	99.9	94.6	99.3
Redundancy	12.6	8.6	12.3
CC(1/2)	0.998	0.999	0.914

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.5M sodium chloride, 0.1M MES pH6.0, 20%PEG6000