7DVJ

Structure of beta-mannanase BaMan113A with mannobiose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-19
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979
Spacegroup name	P 2 21 21
Unit cell lengths	56.493, 110.284, 150.438
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 1.650
R-factor	0.1665
Rwork	0.165
R-free	0.18820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3civ
RMSD bond length	0.011
RMSD bond angle	1.585
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.710
High resolution limit [Å]	1.650	1.650
Number of reflections	108815	10962
<I/σ(I)>	11.8
Completeness [%]	97.9
Redundancy	7.4
CC(1/2)	0.858	0.905

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	tacsimate pH 7.0