7DVE
Crystal structure of FAD-dependent C-glycoside oxidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2019-06-22 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.9000 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 267.081, 267.081, 87.209 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.190 - 2.400 |
| R-factor | 0.2259 |
| Rwork | 0.224 |
| R-free | 0.25720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3pl8 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.980 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | BALBES |
| Refinement software | PHENIX (1.9-4092) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.190 | 2.530 |
| High resolution limit [Å] | 2.000 | 2.400 |
| Rmerge | 0.080 | 0.571 |
| Number of reflections | 90727 | 13294 |
| <I/σ(I)> | 12.42 | 3.26 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.5 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 30%(w/v) PEG4000, 0.1 M Tris-HCl pH8.5, 0.2 M Lithium Sulfate |
