7DVA
Structure of wild type Bt4394, a GH20 family sulfoglycosidase, in complex with 6S-GlcNAc
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-04-28 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979191 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.500, 125.596, 84.640 |
Unit cell angles | 90.00, 90.63, 90.00 |
Refinement procedure
Resolution | 50.483 - 1.550 |
Rwork | 0.142 |
R-free | 0.18440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3rcn |
RMSD bond length | 0.005 |
RMSD bond angle | 1.217 |
Data reduction software | XDS |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.190 | 1.580 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.097 | 1.005 |
Number of reflections | 145268 | 7086 |
<I/σ(I)> | 11.5 | 2 |
Completeness [%] | 97.4 | |
Redundancy | 6.8 | |
CC(1/2) | 0.996 | 0.584 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 282 | 10 mM of 4MU-6S-GlcNAc with 10 mg/mL protein in the buffer of 25 mM PH 8.0, =300 mM NaCl was mixed with 0.1 M BICINE, pH 8.5, 20 % (w/v) PEG 10000 at 1:1 to carry on co-crystallization. |