7DV8

The crystal structure of rice immune receptor RGA5-HMA2.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 1 2 1
Unit cell lengths	119.033, 78.230, 121.004
Unit cell angles	90.00, 90.54, 90.00

Refinement procedure

Resolution	43.994 - 2.447
R-factor	0.2069
Rwork	0.206
R-free	0.24710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5zne
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.000	2.530
High resolution limit [Å]	2.440	2.450
Rmerge	0.083
Rmeas	0.091
Rpim	0.036
Number of reflections	79835	4041
<I/σ(I)>	29.41
Completeness [%]	96.8
Redundancy	6.4
CC(1/2)	0.994	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	PEG 3350, ammonium nitrate