7DV8
The crystal structure of rice immune receptor RGA5-HMA2.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-05-29 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 2 1 |
Unit cell lengths | 119.033, 78.230, 121.004 |
Unit cell angles | 90.00, 90.54, 90.00 |
Refinement procedure
Resolution | 43.994 - 2.447 |
R-factor | 0.2069 |
Rwork | 0.206 |
R-free | 0.24710 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5zne |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.000 | 2.530 |
High resolution limit [Å] | 2.440 | 2.450 |
Rmerge | 0.083 | |
Rmeas | 0.091 | |
Rpim | 0.036 | |
Number of reflections | 79835 | 4041 |
<I/σ(I)> | 29.41 | |
Completeness [%] | 96.8 | |
Redundancy | 6.4 | |
CC(1/2) | 0.994 | 0.990 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 291 | PEG 3350, ammonium nitrate |