7DUV
Structure of Sulfolobus solfataricus SegB protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL15A1 |
Synchrotron site | NSRRC |
Beamline | BL15A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2020-02-28 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.993 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 61.773, 61.773, 171.925 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.070 - 3.200 |
R-factor | 0.2849 |
Rwork | 0.280 |
R-free | 0.32570 |
Structure solution method | MAD |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AutoSol |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 3.310 |
High resolution limit [Å] | 3.200 | 6.870 | 3.200 |
Rmerge | 0.158 | 0.035 | 1.120 |
Rmeas | 0.164 | 0.037 | 1.171 |
Rpim | 0.046 | 0.011 | 0.333 |
Total number of observations | 72822 | ||
Number of reflections | 5959 | 670 | 546 |
<I/σ(I)> | 6.4 | ||
Completeness [%] | 99.1 | 97.5 | 96.5 |
Redundancy | 12.2 | 10.9 | 10.5 |
CC(1/2) | 1.000 | 0.962 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | ammonium sulfate, PEG 4000 |