7DUP
Apo structure of wild type Bt4394, a GH20 family sulfoglycosidase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-12-09 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979183 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 66.585, 63.001, 78.690 |
Unit cell angles | 90.00, 111.34, 90.00 |
Refinement procedure
Resolution | 73.290 - 1.620 |
Rwork | 0.145 |
R-free | 0.18850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3rcn |
RMSD bond length | 0.006 |
RMSD bond angle | 1.232 |
Data reduction software | XDS |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 73.290 | 1.650 |
High resolution limit [Å] | 1.620 | 1.620 |
Rmerge | 0.091 | 0.594 |
Number of reflections | 76353 | 3495 |
<I/σ(I)> | 12.6 | 2 |
Completeness [%] | 99.0 | |
Redundancy | 5.9 | |
CC(1/2) | 0.997 | 0.682 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 282 | PEG 10000, MES, Cl |