7DUO

Crystal structure of daratumumab fab and CD38 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-16
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9798
Spacegroup name	P 1 2 1
Unit cell lengths	81.611, 54.658, 93.052
Unit cell angles	90.00, 98.16, 90.00

Refinement procedure

Resolution	46.055 - 2.810
Rwork	0.231
R-free	0.23760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yh3
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.810	2.810
Rmerge	0.183	0.183
Number of reflections	19416	19416
<I/σ(I)>	12.18
Completeness [%]	100.0
Redundancy	6.4
CC(1/2)	0.969	0.969

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION, RECRYSTALLIZATION		291	0.2M Sodium phosphate monobasic monohydrate, 20% w/v Polyethylene glycol 3350