7DUL
Crystal structure of the Thermus thermophilus (HB8) 30S ribosomal subunit with mRNA and cognate transfer RNA anticodon stem-loop and sisomicin derivative N3''MS bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-06-19 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9898 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 400.830, 400.830, 174.670 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.884 - 3.620 |
R-factor | 0.183 |
Rwork | 0.183 |
R-free | 0.22000 |
Structure solution method | FOURIER SYNTHESIS |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (DEV-3318) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 39.884 | 39.884 | 2.990 |
High resolution limit [Å] | 2.920 | 13.050 | 2.920 |
Rmerge | 0.860 | 0.066 | |
Rmeas | 0.865 | 0.066 | |
Total number of observations | 24751080 | ||
Number of reflections | 297269 | 3583 | 15706 |
<I/σ(I)> | 6.09 | 68.65 | |
Completeness [%] | 97.5 | 95.1 | 70.2 |
Redundancy | 83.262 | 88.497 | 26.516 |
CC(1/2) | 0.999 | 1.000 | 0.368 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 17% MPD |