7DUK

Crystal structure of the Thermus thermophilus (HB8) 30S ribosomal subunit with mRNA and cognate transfer RNA anticodon stem-loop and sisomicin derivative N1,3''MS bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-19
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9898
Spacegroup name	P 41 21 2
Unit cell lengths	400.830, 400.830, 174.670
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.884 - 3.600
R-factor	0.1862
Rwork	0.186
R-free	0.22800
Structure solution method	FOURIER SYNTHESIS
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (DEV-3318)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.884	39.884	2.990
High resolution limit [Å]	2.920	13.040	2.920
Rmerge	1.406	0.057
Rmeas	1.415	0.057
Total number of observations	26452135
Number of reflections	292971	3546	12235
<I/σ(I)>	7.54	85.07
Completeness [%]	95.9	94.1	54.6
Redundancy	90.289	88.979	40.702
CC(1/2)	0.995	1.000	0.420

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	17% MPD